TY  - JOUR
T1  - Local temperature control device using a transparent electrode in the viewing area of a microscope: development and application to RT-PCR
A1  - Oshige, Masahiko
A1  - Yokosawa, Ryo
A1  - Shinohara, Hitomi
A1  - Suzuki, Maho
A1  - Katsura, Shinji
Y1  - 2022///
KW  - Indium tin oxide (ITO) electrode
KW  - temperature sensor
KW  - temperature control
KW  - polymerase chain reaction (PCR).
JF  - International Journal of Plasma Environmental Science and Technology
VL  - 16
IS  - 3
SP  - e03005
EP  - e03005
DO  - 10.34343/ijpest.2022.16.e03005
UR  - http://ijpest.com/Contents/16/3/e03005.html
N2  - The reaction temperature plays a key role in observing biochemical reactions through a microscope. In conventional methods, the actual local temperature of the observed area is different from the measured local temperature because the heater and the temperature sensor are far apart. The electrical resistance of indium tin oxide, a transparent electrode, changes with its temperature. Based on this property, we developed a temperature control system for the viewing area of a microscope. In this system, we heated the transparent electrode by Joule heating, and temperature measurement is conducted on the same electrode in a time-sharing manner. Then, we applied this system to temperature cycles for a reverse transcription-polymerase chain reaction in emulsions. Successful DNA amplification was confirmed by observing the fluorescence of FAM, whose quenching was terminated via oligonucleotide cleavage by DNA amplification in the emulsions.
ER  -